| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 211 | Clostridium tepidum | TTTGCGGAACCACTGGAGA | TCTGCCCATTTGCTCATAGCT | 59.17 | 59.79 | 157 | 92 |
100.00%
|
100.00%
|
| Primer 212 | Clostridium tepidum | AAGCGCAGGTGCAGATGT | TGCTGTAGCTCCTGCCAT | 59.97 | 58.27 | 241 | 92 |
100.00%
|
100.00%
|
| Primer 213 | Clostridium tepidum | GCATCGGCACTGACAGATGT | TGCCAACTCCCTTACCAGG | 60.74 | 58.93 | 264 | 92 |
100.00%
|
100.00%
|
| Primer 214 | Clostridium tepidum | GCATCGGCACTGACAGATGT | TTGCCAACTCCCTTACCAGG | 60.74 | 59.60 | 265 | 92 |
100.00%
|
100.00%
|
| Primer 215 | Clostridium tepidum | AGCTGGTAAGGGGTGTGAG | TCCACCAGAAACAGCTACAGC | 58.62 | 60.27 | 178 | 92 |
100.00%
|
100.00%
|
| Primer 216 | Clostridium tepidum | AGGAATGGGAAATGGCGACTT | GCTGCAGCTATAAAAGGTCCA | 59.99 | 58.36 | 183 | 92 |
100.00%
|
100.00%
|
| Primer 217 | Clostridium tepidum | GGAATGGGAAATGGCGACTT | GCTGCAGCTATAAAAGGTCCA | 58.53 | 58.36 | 182 | 92 |
100.00%
|
100.00%
|
| Primer 218 | Clostridium tepidum | GGGGAATGGGAGAAACAGC | TGCCCTTCGTGCTCATGT | 58.11 | 59.25 | 157 | 92 |
100.00%
|
100.00%
|
| Primer 219 | Clostridium tepidum | AGGCCATTTTGGAAGTGGGA | GCTCCTTGATCATCACCGC | 59.51 | 58.68 | 282 | 92 |
100.00%
|
100.00%
|
| Primer 220 | Clostridium tepidum | TAGGTCCAGCCTCAGCAGT | TGCTCCATGTATAGGTGCCG | 59.92 | 59.61 | 185 | 91 |
100.00%
|
100.00%
|